reaction mixture contained nebuffer 2 1 (New England Biolabs)
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reaction mixture contained nebuffer 2 1
Reaction Mixture Contained Nebuffer 2 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 906 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nebuffer+2/NEBuffer+1/pm42122073-97-1-4
Average 97 stars, based on 906 article reviews
Reaction Mixture Contained Nebuffer 2 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 906 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nebuffer+2/NEBuffer+1/pm42122073-97-1-4
Average 97 stars, based on 906 article reviews
reaction mixture contained nebuffer 2 1 - by Bioz Stars,
2026-09
97/100 stars
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other:Article Title: A novel amplification strategy based on enzyme-assisted hairpin-mediated entropy-driven catalysis for sensitive detection of MicroRNA-21 Article Snippet: MicroRNA-21 (miRNA-21) has been identified as an important biomarker for cancer diagnosis.. Herein, a novel amplification strategy based on an enzyme-assisted hairpin-mediated entropy-driven catalysis (EA-HEDC) is proposed for the precise identification of miRNA-21.. We have designed the locked hairpin DNA probe (LH1) rationally, miRNA-21 recognizes LH1 and initiates the hairpin-mediated entropy-driven catalysis 1 (HEDC1) reaction. Ligation:Article Title: Expanding the chemical diversity of RNA by transcriptional incorporation of amino acid- and glycosyl-modified nucleotides Article Snippet: .. To generate the 5′-monophosphates required for downstream ligation, purified RNA transcripts were incubated with RNA 5′ Pyrophosphohydrolase (2.5 U/μg RNA) in 1× Purification:Article Title: Expanding the chemical diversity of RNA by transcriptional incorporation of amino acid- and glycosyl-modified nucleotides Article Snippet: .. To generate the 5′-monophosphates required for downstream ligation, purified RNA transcripts were incubated with RNA 5′ Pyrophosphohydrolase (2.5 U/μg RNA) in 1× Incubation:Article Title: Expanding the chemical diversity of RNA by transcriptional incorporation of amino acid- and glycosyl-modified nucleotides Article Snippet: .. To generate the 5′-monophosphates required for downstream ligation, purified RNA transcripts were incubated with RNA 5′ Pyrophosphohydrolase (2.5 U/μg RNA) in 1× Article Title: Cooperative Roles of Pds5a and Pds5b Constrain Long‐Range Chromatin Interactions in Vertebrate Embryos Article Snippet: Cells were thawed on ice, washed with PBS, and resuspended in 250 μL of ice‐cold Hi‐C lysis buffer (10 mM Tris–HCl, pH 8.0, 10 mM NaCl, 0.2% Igepal CA‐630) containing 50 μL of protease inhibitors (Sigma‐Aldrich, P8340) and incubated on ice for 20 min. .. The lysate was centrifuged at 2500 g for 5 min, washed once with ice‐cold Hi‐C lysis buffer, resuspended in 50 μL of 0.5% SDS, and incubated at 62°C for 10 min. After adding 145 μL of water and 25 μL of 10% Triton X‐100, samples were incubated at 37°C for 15 min. Next, 25 μL of 10× Lysis:Article Title: Cooperative Roles of Pds5a and Pds5b Constrain Long‐Range Chromatin Interactions in Vertebrate Embryos Article Snippet: Cells were thawed on ice, washed with PBS, and resuspended in 250 μL of ice‐cold Hi‐C lysis buffer (10 mM Tris–HCl, pH 8.0, 10 mM NaCl, 0.2% Igepal CA‐630) containing 50 μL of protease inhibitors (Sigma‐Aldrich, P8340) and incubated on ice for 20 min. .. The lysate was centrifuged at 2500 g for 5 min, washed once with ice‐cold Hi‐C lysis buffer, resuspended in 50 μL of 0.5% SDS, and incubated at 62°C for 10 min. After adding 145 μL of water and 25 μL of 10% Triton X‐100, samples were incubated at 37°C for 15 min. Next, 25 μL of 10× |
